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2.79J/3.96J/20.441/HST522J
BIOMATERIALSBIOMATERIALS-TISSUE INTERACTIONS:
Tools for Understanding the M l l C ll l T l f U d di h Molecular, Cellular, and Physiological Bases of the Tissue Response to Implants p
BIOMATERIALS-TISSUE INTERACTIONS
Tissue* + Biomaterial**
Cell
+ Matrix**
CELL-MATRIX INTERACTIONS
In Tissu Tissue Cell + Extracellular Matrix In Tissue Engineering Scaffolds Cell + Biomaterial Scaffold
CONCEPTS FOR UNDERSTANDING BIOMATERIALS-TISSUE INTERACTIONS Control Volume Unit Cell Processes Types of Tissue Tissues Tissue Formation and Remodeling In Vitro Wound H li I Vi W d Healing In Vivo
Articular Cartilage
Extracellular Extracellular
Matrix
Cell
4 mm
10 m
40min
2 3
Control Volume
4
B. Kinner
Zaleskas, J. M., et al. "Contractile forces generated by articular chondrocytes in collagen-glycosaminoglycan matrices." Biomaterials 25 no. 7-8 (2004):1299-1308.
Cell + Matrix Matrix Cell Product + Soluble (Regulator) Regulator B (Regulator) Product
Control Volume
Autocrine factor
Cell + Matrix Cell + Matrix Product + Soluble (Regulator) (Regulator) Regulator Product
Control Volume Another Cell; Circulation; Paracrine Endocrine
Cell + Matrix Cell + Matrix Product + Soluble (Regulator) Regulator B (Regulator) Product
Control Volume
CONCEPTS FOR UNDERSTANDING BIOMATERIALS-TISSUE INTERACTIONS Control Volume Unit Cell Processes Types of Tissue Tissues Tissue Formation and Remodeling In Vitro Wound H li I Vi W d Healing In Vivo
COLLAGEN-GAG MATRICES: MODEL BIOMATERIALS (ANALOGS OF EXTRACELLULAR MATRIX) Investigation of cell interactions (UCPs) in vitro Type I (bovine and porcine) T Type II ( (porcine) i ) Chondroitin 6-sulfate
500m
Can provide insights into cell behavior in vivo. Can provide insights into scaffold composition and structure for improved p p performance in regenerative medicine.
J. Cheng
J. Cheng
DN (ug NA g)
40 30 20 10 0 0 0 10 10 20 20
30 30
Day
100 m
2001 L. C. Santy and J. E. Casanova. License CC BY-NC-SA. Published Published by The Rockefeller University Press Rockefeller University Press. http://dx.doi.org/10.1083/jcb.200104019
40min
J. Zaleskas, et al., Biomat. 25:1299 (2004)
2 3
4 5
B. Kinner
Diagram removed due to copyright restrictions. Fig. i Mad i KidneyIInt. 41 (1992): 562. Fi 2 inM dri, Kid (1992) 562 Schematic of the modulation of microvascular endothelial cell phenotype during angiogenesis.
0.1
0.02
0.05
0.01
0 2 7 15 29
0 2 7 15 29
Day
Day
CR Lee, et al., Biomat. 2001;22:3145
-smooth muscle actin-fusion peptide (SMA-FP) inhibits the tension exerted by lung fibroblasts on silicone substrates. After washing our of the FP, cells contract again.
40 min
Courtesy Elsevier, Inc., http://www.sciencedirect.com. Used with permission.
B Kinner
40 min 40 min
300 min
Courtesy Elsevier, Inc., http://www.sciencedirect.com. Used with permission.
10mm 10 10mm
Non-Seeded and Cell-Seeded Collagen-GAG Scaffolds
21 days
S. Vickers
Adult canine articular chondrocytes (passage 3)3) contract type I I Adult canine articular chondrocytes (passage contract a a type collagen-GAG matrix, reflected inin the decrease in diameter collagen-GAG matrix, reflectedContraction g Cell-Mediated the decrease in diameter , Cell Mediated
100
Mean SEM
90 80 70 60 50 40 30 0 10
Day
20
30
Chondrocytes express the gene for -smooth muscle actin and this enables them to contract
Courtesy of John Wiley and Sons, Inc. Used with permission. Source: Kinner, B., and M. Spector. J Orthop Res 19 (2001): 233-241.
Neg. control
Courtesy of John Wiley and Sons, Inc. Used with permission. Fig 1a and b in Kim, A. C., and M. Spector. "Distribution of Chondrocytes Containing Alpha-smooth Muscle Actin in Human Articular Cartilage." J Orthop Res 18 (2000): 749.
MUSCULOSKELETAL CELLS THAT CAN EXPRESS a a-SMOOTH MUSCLE ACTIN AND CAN CONTRACT
Articular chondrocyte Osteoblast Meniscus fibroblast and fibrochondrocyte Intervertebral disc fibroblast and fibrochondrocyte Ligament fibroblast Tendon fibroblast Synovial cell M. Spector, Mesenchymal stem cell esenchymal stem cell Mesenchymal Wound Repair Regen.
9:11-18 (2001) 9:11-
BIOMATERIALS-TISSUE INTERACTIONS
Cell C ll + Matrix M t i
Connective Tissue Epithelia Muscle M l Nerve
Control Volume
BIOMATERIALS-TISSUE INTERACTIONS
Cell + Matrix
Connective Tissue Epithelia Muscle Nerve N
BIOMATERIALS-TISSUE INTERACTIONS
Cell + Matrix
Connective Tissue Epithelia Muscle Nerve Adhesion Protein Collagen Biomaterial
Integrin
Photos removed due to copyright restrictions. Figure 3 in Watters, C. Cell Biol. Ed. 2:210 (2003) Image and links to associated videos at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC256980/figure/F3/
Reduction of migration speed and induction of detached, nonmobile spherical morphology by adhesion perturbing anti1 i t i t bi ti 1 integrin antibody, as a consequence of impaired collagen fibril binding.
Cell + Matrix
Connective Tissue Epithelia Muscle Nerve
Cell + Matrix
Connective Tissue Epithelia Muscle Nerve N
Product
Cell proliferation Matrix molecules, , enzymes, cytokines Migration Translocation Contraction Strain Endocytosis Solubilized fragments Exocytosis Regulators
Cell + Matrix
Connective Tissue Epithelia Muscle N Nerve
Product + Regulator
Mitosis Synthesis Migration Contraction Endocytosis Exocytosis Cytokines (Growth Factors)
REGULATORS
Cytokines/Growth Factors
http://themedicalbiochemistrypage.org/growthhttp://themedicalbiochemistrypage.org/growthfactors.html (previously: http://web.indstate.edu/thcme/mwking/growthhttp://web.indstate.edu/thcme/mwking/growthfactors.html
http://www.copewithcytokines.de/
Cell Ce + Matrix at
Connective Adhesion Mitosis Tissue ssue Protein Synthesis Epithelia Collagen Migration Muscle Biomaterial Contraction Endocytosis Nerve Integrin Exocytosis
Cell Ce + Matrix at
Connective Adhesion Mitosis Tissue ssue Protein Synthesis Epithelia Collagen Migration Muscle Biomaterial Contraction Endocytosis Nerve Integrin Exocytosis
Cell + Matrix
Product + Regulator
Matrix strain Cytokines Mitosis Connective Adhesion Synthesis (contracture/ (Growth Factors) Tissue Protein g ) Migration shrinkage) Epithelia E ith li Collagen Muscle Biomaterial Contraction Endocytosis Nerve Exocytosis Integrin
TGFTGF-1 G
Contraction
Fibroblast + Collagen
Contracture + Reg.
CONCEPTS FOR UNDERSTANDING BIOMATERIALS - TISSUE INTERACTIONS Control Volume Unit Cell Processes Types of Tissue Tissues Tissue Formation and Remodeling In Vitro Wound H li I Vi W d Healing In Vivo
+FGF-2
+FGF-2
Canine chondrocytes grown in a type II collagen-GAG scaffold for 2 weeks. (Safranin O stain for GAGs)
CONCEPTS FOR UNDERSTANDING BIOMATERIALS-TISSUE INTERACTIONS Control Volume Unit Cell Processes Types of Tissue Tissues Tissue Formation and Remodeling In Vitro Wound H li I Vi W d Healing In Vivo
Granulation Tissue
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