Chelsea Behnke Hour 4 1/2/12 Daphnia Lab Report I.Title Affects Different Drugs Have on the Heart II.

Background Daphnia are tiny crustaceans that live in most freshwater sources. They are not quite microscopic but they are just big enough to see their internal organs. Their skin is made up of a clear layer so its easy to see directly through them Daphnia are commonly used to test the effects of different substances and how they make the heart react. Stimulants are used to increase heart rate and depressants have the opposite effect. I fact, depressants lower the heart rate. III.Purpose To determine how common drugs affect heart rates IV.Hypothesis If we add the different drugs to the daphnia then the heart rates will increase from the normal heart rate because thats how each drug is meant to affect the rates to control the change of what is wrong original with the body. V.Materials VI.Procedure Daphnia Clean slides Cover slip Microscope Dropper Spring Water Caffeine Aspirin Sleeping Aid Nicotine Alcohol

1. Place a Daphnia into the well of a clean slide 2. Add a small drop of the drug you are testing into the well with the daphnia and place a vocer slip over the slide 3. Time one minute, then put daphnia under microscope and turn the light on to see the heartbeat 4. Find the heart and start counting it ten seconds 5. Complete three trials with 10 second intervals in which you count the Daphnias heart rate and record that number in the data table. 6. Repeat the steps above with all the different drugs that are being tested.( Do not have the light on in between trials because the daphnia will die)

VII.Data

Drug 1. Spring Water (control) 2. Caffeine 3. Aspirin 4. Alcohol 5. Sleep Aid 6. Nicotine

My Results 198 228 254 206 214 140

Class Averages 220.8 242.3 180.3 179.1 158.3 233.1

Nicotine

Sleep Aid

Alcohol Class Aspirin Personal

Caffeine

Spring Water 0 50 100 150 200 250 300

VIII.Analysis According to the data collected, the aspirin, and caffeine increased the heart rate while, the alcohol, sleep aid, and nicotine all slowed the heart rate. The alcohol should have increased it dramatically, in which it didnt, which means there was room for a source of error. IX.Conclusion Through all of this my hypothesis was not totally supported. Some of the drugs did increase the heart rate but there should have been only two that slowed it down because they were depressants, not stimulants. There was definitely a lot of room for error in this experiment because if the light was left on

or the time went to long then the daphnia could die or adapt to the drug. Another place where error could have occurred was if the slides werent fully clean and the droppers got cross contaminated with multiple drugs. All of these results and tests can determine how much of a certain drug to give a person or what type of effects these different drugs have. This is an easy way to find new drugs and an even better way to experiment with them without causing a great deal of damage.