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Isoelectric point (pI) pI = "isoelectric pH" = "isoelectric point" = pH at which the NET charge on a molecule is ZERO .

. o If pH < pI, net charge is positive (more + than - charges)

o If pH > pI, net charge is negative (more - than + charges)

pI = the pH exactly halfway between the two pK a values surroundingthe zero net charge equivalence point on the titration curve(examples to be analyzed in class: Gly and His)

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