Professional Documents
Culture Documents
LOGO
OBJETIVOS
Conocer las tcnicas instrumentales documentadas hasta la actualidad para la determinacin de Capsaicinoides
CONTENIDO
1 2 INTRODUCCIN TCNICAS INSTRUMENTALES
3
4 5
COMPARACIONES
CONCLUSIONES BIBLIOGRAFA
ORIGEN
INTRODUCCIN
Genero: Capsicum
GENERALIDADES
INTRODUCCIN
Al igual que cualquier alimento, hay una gama de compuestos qumicos que le confieren sus caractersticas propias.
COMPOSICIN
Capsicum
INTRODUCCIN
Capsaicinoides:
Capsinoides (CLS):
CAPSAICINOIDES
INTRODUCCIN
fenilalquilamidas
CAPSAICINOIDES
INTRODUCCIN
DISTRIBUCIN
INTRODUCCIN
<1.0% w/w
10
DISTRIBUCIN
INTRODUCCIN
90%
11
EFECTOS Y APLICACIONES
INTRODUCCIN
1. Antioxidantes
12
EFECTOS Y APLICACIONES
INTRODUCCIN
3. Anti-inflamatorios
4. Anti-microbiano
13
EFECTOS Y APLICACIONES
INTRODUCCIN
5. Quema Grasa!!!
6. Armas No Letales
14
IMPORTANCIA
INTRODUCCIN
Todos
Uso
Tcnicas Instrumentales para la Determinacin de Capsaicinoides
CAP.
15
TCNICAS ESTUDIADAS
CG
CLAR
Electroqumicas
VOC
Tcnicas Instrumentales para la Determinacin de Capsaicinoides
16
TCNICAS INSTRUMENTALES
17
PRE-TRATAMIENTO
PMDS/DVB
TCNICAS INSTRUMENTALES
18
TCNICAS INSTRUMENTALES
19
DETECCIN
TCNICAS INSTRUMENTALES
CG
20
FRAGMENTACIN
TCNICAS INSTRUMENTALES
21
CROMATOGRAMAS CG-EM
TCNICAS INSTRUMENTALES
22
23
CLAR-DETECTORES
UV
TCNICAS INSTRUMENTALES
DEQ
DAD
EM
Tcnicas Instrumentales para la Determinacin de Capsaicinoides
24
ESPECTROFOTOMTRICOS
TCNICAS INSTRUMENTALES
0,6
0,5
0,4
0,3
0,2
0,1
Grupo Cromforo
25
CLAR-DETECTORES
UV
TCNICAS INSTRUMENTALES
DEQ
DAD
EM
Tcnicas Instrumentales para la Determinacin de Capsaicinoides
26
ELECTROQUMICAMENTE
TCNICAS INSTRUMENTALES
Grupo Electroactivo
Tcnicas Instrumentales para la Determinacin de Capsaicinoides
27
TCNICAS INSTRUMENTALES
TCNICAS INSTRUMENTALES
29
COMPORTAMIENTO ELECTROQUMICO
1
TCNICAS INSTRUMENTALES
I
1 2 3
R
4 3 4
Tcnicas Instrumentales para la Determinacin de Capsaicinoides
30
DE VOC AL DETECTOR
E Q
TCNICAS INSTRUMENTALES
I
Proceso EQE
31
TCNICAS INSTRUMENTALES
32
CLAR-DETECTORES
UV
TCNICAS INSTRUMENTALES
DEQ
DAD
EM
Tcnicas Instrumentales para la Determinacin de Capsaicinoides
33
CLAR-UV
COMPARACIN INSTRUMENTAL
34
CLAR-EM
COMPARACIN INSTRUMENTAL
35
UV Vs. DEQ!!!
60
COMPARACIN INSTRUMENTAL
40
20
-20 0 2 4 6 8 10 12 14
36
UV Vs. DEQ!!!
600
COMPARACIN INSTRUMENTAL
CAP
400
200
-200
-400
DEQ +450mV
140
37
PARMETROS DE SENSIBILIDAD
COMPARACIN INSTRUMENTAL
COMPARACIN INSTRUMENTAL
39
COMPARACIN INSTRUMENTAL
COMPARACIN DE TCNICAS
COMPARACIN INSTRUMENTAL
41
CONCLUSIONES
42
CONCLUSIONES
43
BIBLIOGRAFA
Electroqumica : 1. Roohollah Torabi Kachoosangi, Gregory G. Wildgoose and Richard G. Compton Carbon nanotube-based electrochemical sensors for quantifying the heat of chilli peppers: the adsorptive stripping voltammetric determination of capsaicin Analyst, 2008, 133, 888895 2. Yavuz Yardim. Sensitive Detection of Capsaicin by Adsorptive Stripping Voltammetry at a Boron-Doped Diamond Electrode in the Presence of Sodium Dodecylsulfate Electroanalysis 2011, 23, No. 10, 2491 2497 3. Veronika Supalkova, Ales Horna, Helena Stavelikova, Ladislav Havel. Study of Capsaicin Content in Various Parts of Pepper Fruit by Liquid Chromatography with Electrochemical Detection Acta Chim. Slov. 2007, 54, 5559 4. Yu Ya, Leixing Mo, Tianshun Wang. Highly sensitive determination of capsaicin using a carbon paste electrode modied with amino-functionalized mesoporous silica Colloids and Surfaces B: Biointerfaces (2012) Articulo Aceptado para publicacin
44
BIBLIOGRAFA
Tcnicas Cromatogrficas: 1. Gerardo F. Barbero, Miguel Palma, Carmelo G. Barroso Determination Of Capsaicinoids In Peppers By Microwave Assisted Extractionhigh-performance Liquid Chromatography With uorescence Detection. Analytica Chimica Acta 578 (2006) 227233 2. Gerardo F. Barbero, Miguel Palma, Carmelo G. Barroso Fast Determination Of Capsaicinoids From Peppers By High-performance Liquid Chromatography Using A Reversed Phase Monolithic Column Food Chemistry 107 (2008) 12761282 3. Irena Perucka, Wies Aw Oleszek Extraction And Determination Of Capsaicinoids In Fruit Of Hot Pepper Capsicum Annuum L. By Spectrophotometry And High-performance Liquid Chromatography Food Chemistry 71 (2000) 287291 4. A.L. Kurian And A.N. STARKS HPLC Analysis Of Capsaicinoids Extracted From Whole Orange Habaero Chili Peppers Journal Of Food Science
45
BIBLIOGRAFA
5. Ana Garce S-claver, Maria S. Arnedo-andre S, Javier Abadia. Determination Of Capsaicin And Dihydrocapsaicin In Capsicum Fruits By Liquid Chromatography-electrospray/Time-of-flight Mass Spectrometry J. Agric. Food Chem., Vol. 54, No. 25, 2006 6. Jochen Pauwels, Ward Dautry, Larissa Van Den Bossche. Optimization And Validation Of Liquid Chromatography And Headspace-gas Chromatography Based Methods For The Quantitative Determination Of Capsaicinoids, Salicylic Acid, Glycol Monosalicylate, Methyl Salicylate, Ethyl Salicylate, Camphor And L-menthol In A Topical Formulation Journal of pharmaceutical and biomedical analysis 60 (2012) 5158 7. Araceli Pea-alvarez, Erika Ramrez-maya, Lus ngel Alvarado-surez. Analysis Of Capsaicin And Dihydrocapsaicin In Peppers And Pepper Sauces By Solid Phase Microextractiongas Chromatographymass Spectrometry. J. Chromatogr. A 1216 (2009) 28432847
46
LOGO
LOGO
www.themegallery.com
TCNICAS DE IONIZACIN
TCNICAS DE IONIZACIN
POLARIDAD VS IONIZACIN
www.themegallery.com
www.themegallery.com
ANALIZADORES
ASISTENCIA COMPUTACIONAL
www.themegallery.com
Sensibilidad
LOD 3 Sbl/m
LOQ 10 Sbl/m
63
CAPSAICINA
TOXICIDAD CAP
(4,5-dimetoxibencil)-4-meiloctamida DMBMO
CLAR-MS
COMPOSICIN QUMICA
Son Amidas
Son los responsables del Sabor Picante
2. COMPOSICIN QUMICA
Variedad
Poco Picantes Picantes Suaves Fuertemente Picantes
[Capsaicinoides]
0.003-0.01% 0.01-0.3% >0.3%
70
3. CAPSAICINA
Biosintesis
4. ELECTROQUIMICA
Como Ocurre???
72
4. ELECTROQUIMICA
Como Ocurre???
73
GC HPLC
74
CLAR-DETECTORES
Ultravioleta (UV)
75
CLAR-DETECTORES
76
CLAR-DETECTORES
Deteccin Electroqumica (ECD)
77
Interface
78
79